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Materials and methods Cell culture and receptor gene
2019-10-08
Materials and methods Cell culture and receptor gene transfection. PC12 SF1670 clinical were purchased from the Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, China. The cDNA for mouse CysLT1 or CysLT2 receptor (mCysLT1 and mCysLT2, subcloned into pcDNA3.0) was kindly gifted by
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IL is not expressed in tissues constitutively because of its
2019-10-08
IL-8 is not expressed in tissues constitutively because of its strong chemoattractant, proinflammatory, and angiogenic activities. Rather, it is an inducible factor, produced by a variety of cell types, including leukocytes, fibroblasts, endothelial and epithelial cells, in response to proinflammato
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In order to further evaluate
2019-10-08
In order to further evaluate cpt1a function, we determined its tissue distribution in large yellow croaker. The mRNA levels of cpt1a were detected in all the tested tissues, but at varying levels. cpt1a mRNA expression was the highest in liver among different tissues, similar to reports in other fis
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br Thermodynamic modeling br Selection of data The
2019-10-08
Thermodynamic modeling Selection of data The solutes were selected based on the set of data needed to evaluate the CPA-EoS for prediction of their solubility in CO2 + cosolvent, as shown in Fig. 1. Critical temperatures, melting temperatures and enthalpies of fusion of the solutes are required
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Inopportunely normal aging brings about decreases
2019-10-08
Inopportunely, normal aging brings about decreases in frontal D1 receptor densities and a gradual decline in postsynaptic markers of dopamine in striatal, neocortical, and limbic areas of the Oligomycin Complex (Bäckman et al., 2006, de Keyser et al., 1990, Karrer et al., 2017, Suhara et al., 1991,
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The N B method has the
2019-10-07
The N&B method has the overall advantage that it is not restricted to certain dyes specific for energy transfer [55], and is also able to detect higher order receptor c-Myc tag on the focal point in living cells. Conclusion The N&B method has shown to be a powerful tool to locate and quantify th
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NF B is a family of protein
2019-10-07
NF-κB is a family of protein mediators that regulate various innate and adaptive immune responses [29,30]. The NF-κB family consists of the following five proteins: c-Rel (Rel); p65 (RelA); RelB; p50(NF-κB1); and p52(NF-κB2). It has been confirmed that NF-κB is activated by TNF family cytokines, suc
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br Abbreviations br Acknowledgements br Introduction Dopamin
2019-10-07
Abbreviations Acknowledgements Introduction Dopamine β-hydroxylase (DbH) is the enzyme catalysing the conversion of dopamine to norepinephrine, two important neurotransmitters involved in the central nervous system [1]. This enzyme has been isolated from bovine chromaffin granules as a tetr
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br Conformational properties of DGK br Interaction
2019-10-07
Conformational properties of DGKϵ Interaction of DGKϵ with its lipid substrate A major component of the interaction between DGKϵ and lipid bilayers is the insertion of the hydrophobic segment comprising residues 20–42 into the membrane, as discussed above. However, this is not the only interac
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In the PTEN loss mouse model deletion of ATG gene
2019-10-07
In the PTEN loss mouse model, deletion of ATG7 gene not only caused an autophagy-deficient phenotype, but also postponed the progression of inoculated prostate tumor [22]. On the basis of these experimental facts, these ATG7 and PTEN related genes were then compared with the 3020 BCR-associated gene
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This review focuses on the atomic basis of CRM mediated
2019-10-07
This review focuses on the atomic basis of CRM1-mediated nuclear export. There are now 27 crystal structures of CRM1 in the Protein Data Bank (PDB) (www.rcsb.org) [64]. Collectively, this large body of work explains various aspects of CRM1 function. Here we summarize the structure–function studies t
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However one major challenge will be to
2019-10-07
However, one major challenge will be to understand how the diverse actions of D2Rs in multipartite, dopamine-sensitive circuits are coordinated to produce specific behaviors across species. It is possible that emerging techniques for imaging neuronal activity in different neuronal population in beha
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Interestingly selective ETA blockade increased
2019-10-07
Interestingly, selective ETA blockade increased cutaneous blood flow as measured by laser Doppler fluxmetry. This is a measure of total blood flow in the different vessels in the skin including venules, Cap Firefly Luciferase mRNA clinical as well as arterioles. This finding is in accordance with t
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All these results indicate that cleaving
2019-10-07
All these results indicate that cleaving embryos possess a regulative mechanism, sensing the number of inner Fmoc-Lys(Boc)-OH and controlling the proportion of conservative and differentiative divisions of blastomeres. The current observations agree with the results from other laboratories and our
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br Materials and methods br Results
2019-10-07
Materials and methods Results and discussion Conclusions In the present study, we have shown the important role of miR-204-5p in C2C12 myoblast differentiation. Overexpression of miR-204-5p inhibited myogenic differentiation and decreased the formation of slow-twitch myofibers, whereas miR-
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